TWO PROTEINS (TCTIA6 AND TCTIA7) INVOLVED IN THE BIOSYNTHESIS OF THIAMINE CODED BY THEOBROMA CACAO L. GENOME

Authors

  • Juliano Oliveira Santana Universidade Estadual de Santa Cruz (UESC), Ilhéus - BA, Brasil. km 16, Rodovia Ilhéus-Itabuna, Salobrinho, CEP 45662-900, Ilhéus, BA – Brasil. Estudante de Doutorado em Genética e Biologia Molecular. http://orcid.org/0000-0003-3395-6618
  • Gonçalo Santos Silva Universidade Estadual de Santa Cruz (UESC), Ilhéus - BA, Brasil. km 16, Rodovia Ilhéus-Itabuna, Salobrinho, CEP 45662-900, Ilhéus, BA – Brasil. Estudante de Doutorado em Genética e Biologia Molecular.
  • Luciano Angelo de Souza Bernardes Universidade Estadual de Santa Cruz (UESC), Ilhéus - BA, Brasil. km 16, Rodovia Ilhéus-Itabuna, Salobrinho, CEP 45662-900, Ilhéus, BA – Brasil. Professor adjunto no Departamento de Ciências Exatas e Tecnológicas (DCET).

DOI:

https://doi.org/10.14450/2318-9312.v29.e3.a2017.pp208-213

Keywords:

cacao, computational biology, genome, protein, thiamine

Abstract

Thiamine functions as a cofactor for activity of various enzymes that act on the metabolism of carbohydrates and amino acids. This study aimed to analyze through bioinformatic techniques the genes responsible for the biosynthesis of thiamine encoded by the genome of Theobroma cacao L. We found two genes, one in chromosome 6 and one in chromosome 7, being appointed as TcTIA6 and TcTIA7. The multiple alignment revealed a strong identity with the Arabidopsis thaliana protein and its direction is destined to the secretory route of the chloroplast. Conserved motifs in the C-terminal region were found, indicating participation in the DNA repair mechanism. Analyzing the dendrogram it was evident that the genes studied had a common ancestor with Gossypium raimondii. The analysis showed that the TcTIA6 and TcTIA7 proteins are probably bifunctional, since they are involved in the biosynthesis of thiamine and stability of the DNA molecule.

Author Biographies

Juliano Oliveira Santana, Universidade Estadual de Santa Cruz (UESC), Ilhéus - BA, Brasil. km 16, Rodovia Ilhéus-Itabuna, Salobrinho, CEP 45662-900, Ilhéus, BA – Brasil. Estudante de Doutorado em Genética e Biologia Molecular.

Farmacêutico, Doutorando e Mestre em Genética e Biologia Molecular, Especialista em Análsies Clínicas. Departamento de Ciências Biológicas, Centro de Biotecnologia e Genética, Laboratório de Proteômica.

Gonçalo Santos Silva, Universidade Estadual de Santa Cruz (UESC), Ilhéus - BA, Brasil. km 16, Rodovia Ilhéus-Itabuna, Salobrinho, CEP 45662-900, Ilhéus, BA – Brasil. Estudante de Doutorado em Genética e Biologia Molecular.

Doutorando e Mestre em Genética e Biologia Molecular, Departamento de Ciências Biológicas, Laboratório de Melhoramento de Plantas.

Luciano Angelo de Souza Bernardes, Universidade Estadual de Santa Cruz (UESC), Ilhéus - BA, Brasil. km 16, Rodovia Ilhéus-Itabuna, Salobrinho, CEP 45662-900, Ilhéus, BA – Brasil. Professor adjunto no Departamento de Ciências Exatas e Tecnológicas (DCET).

Doutorado em Genética e Mestrado em Biociências Aplicadas à Farmácia. Departamento de Ciências Exatas e Tecnológicas, Núcleo de Biologia Computacional e Gestão de Informações Biotecnológicas.

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Published

2017-09-25

How to Cite

Santana, J. O., Silva, G. S., & Bernardes, L. A. de S. (2017). TWO PROTEINS (TCTIA6 AND TCTIA7) INVOLVED IN THE BIOSYNTHESIS OF THIAMINE CODED BY THEOBROMA CACAO L. GENOME. Infarma - Pharmaceutical Sciences, 29(3), 208–213. https://doi.org/10.14450/2318-9312.v29.e3.a2017.pp208-213

Issue

Vol. 29 No. 3 (2017)

Section

Original article

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