UV-VIS SPECTROSCOPY TO DETECTION OF QUERCETIN IN PLANTS BY FORMATION OF QUERCETIN-ALUMINUM COMPLEX

Authors

  • Fabiele Bernardi Instituto Federal do Paraná - IFPR Av. Bento Munhoz da Rocha, s/n, PRT280 – Trevo da Codapar, CEP 85555-000, Palmas, PR, Brasil.
  • Keller Paulo Nicolini Instituto Federal do Paraná - IFPR Av. Bento Munhoz da Rocha, s/n, PRT280 – Trevo da Codapar, CEP 85555-000, Palmas, PR, Brasil.
  • Jaqueline Nicolini Instituto Federal do Paraná - IFPR Av. Bento Munhoz da Rocha, s/n, PRT280 – Trevo da Codapar, CEP 85555-000, Palmas, PR, Brasil.

DOI:

https://doi.org/10.14450/2318-9312.v29.e3.a2017.pp271-276

Keywords:

Natural products, quercetin, antioxidant effect

Abstract

Nine plant samples were studied from complexation reaction that occurs between aluminum from aluminum chloride (AlCl3) and quercetin. The reaction shows yellow solution, which was spectroscopically evidenced by the bathochromic shift from 360-370 nm to 420-430 nm. Four samples of petals were investigated: white and magenta azalea, burnt yellow lily and dandelion; three leaf samples: aloe, orange and lime; and two peel samples: orange and lime fruits. Among the analyzed samples, only azalea (Rhododendron sp.) white and magenta petals had sufficient quercetin concentration to be detected by UV-Vis spectroscopy and analyzed by quercetin-aluminum complex formation. The interaction between aluminum and quercetin showed a stoichiometry of 2:1, with a binding constant of 3.94 ± 0.34 x 103 mol L-1.

Author Biographies

Fabiele Bernardi, Instituto Federal do Paraná - IFPR Av. Bento Munhoz da Rocha, s/n, PRT280 – Trevo da Codapar, CEP 85555-000, Palmas, PR, Brasil.

Bolsista de iniciação científica e aluna do curso Farmácia pelo Instituto Federal do Paraná - IFPR.

Keller Paulo Nicolini, Instituto Federal do Paraná - IFPR Av. Bento Munhoz da Rocha, s/n, PRT280 – Trevo da Codapar, CEP 85555-000, Palmas, PR, Brasil.

Biológo e Químico, doutor em Química e atualmente professor efetivo do Instituto Federal do Paraná - IFPR.

Jaqueline Nicolini, Instituto Federal do Paraná - IFPR Av. Bento Munhoz da Rocha, s/n, PRT280 – Trevo da Codapar, CEP 85555-000, Palmas, PR, Brasil.

É Licenciada em Química (UNICS), mestre em Química (FURB) e Doutora em Química tendo realizado o doutorado sanduíche pela Universidade Federal do Paraná (UFPR) e pela Karlsruher Institüt für Technologie (KIT)/Engler Bunte Institute em Karlsruhe Alemanha em 2010. Atualmente é professora efetiva do Instituto Federal do Paraná -IFPR.

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Published

2017-09-25

How to Cite

Bernardi, F., Nicolini, K. P., & Nicolini, J. (2017). UV-VIS SPECTROSCOPY TO DETECTION OF QUERCETIN IN PLANTS BY FORMATION OF QUERCETIN-ALUMINUM COMPLEX. Infarma - Pharmaceutical Sciences, 29(3), 271–276. https://doi.org/10.14450/2318-9312.v29.e3.a2017.pp271-276

Issue

Vol. 29 No. 3 (2017)

Section

Technical Note

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