COMPARATIVE ANALYSIS OF METHODS FOR HCV SCREENING IN HEMOTHERAPY SERVICES

Authors

  • Gabriela Carboni ULBRA - Universidade Luterana do Brasil - campus Carazinho. Br 285, Km 335. Carazinho, RS 99500-000
  • Renata Eliane Boehm Serviço de Hemoterapia - Hospital de Clínicas de Porto Alegre - UFRGS. Rua Ramiro Barcelos, 2350. Porto Alegre,RS 90035-9003 http://orcid.org/0000-0001-7334-286X
  • Claudete Mistura Doro Hemocentro Regional de Passo Fundo - HEMOPASSO Av. Sete de Setembro, 1055 - Centro, Passo Fundo - RS, 99010-120 http://orcid.org/0000-0002-9255-3408
  • Caroline Tiecher Hemocentro Regional de Passo Fundo - HEMOPASSO Av. Sete de Setembro, 1055 - Centro, Passo Fundo - RS, 99010-120 http://orcid.org/0000-0003-1834-8382
  • Alexandre Ehrhardt ULBRA - Universidade Luterana do Brasil - campus Carazinho. Br 285, Km 335. Carazinho , RS 99500-000 FASURGS - Faculdade Especializada na Área da Saúde do Rio Grande do Sul Rua Angélica Oto, 160. Boqueirão. Passo Fundo, RS 99025-270 http://orcid.org/0000-0002-2434-7215

DOI:

https://doi.org/10.14450/2318-9312.v32.e4.a2020.pp336-343

Keywords:

Hepatitis C, blood safety, techniques for nucleic acid amplification, immunological tests

Abstract

Among the viral hepatitis, hepatitis C (HCV) consists in a significant public health problem and requires the need for screening tests increasingly effective in detecting HCV, increasing the safety of donated blood. This research compared the effectiveness of HCV screening among the methodologies used by the blood center, also checking whether there was a reduction in the immunological window after the introduction of the nucleic acid test (NAT) methodology, conducted through retrospective analysis of serological record sheets of 12,443 potential blood donors screened from July 2014 to June 2015 for the HCV at the Regional Blood Center of Passo Fundo - Hemopasso. Among all possible blood donors, 45 (0.36%) of them  were anti-HCV inconclusive CMIA with undetectable NAT result. Only 7 (0.05%) of them showed anti-HCV reagent by CMIA methodology and detectable NAT. We observed no detection of HCV, by NAT testing, during the immunological window. There were no falsepositive results for CMIA methodology. With the analysis of the results, it was observed that the CMIA and NAT methodologies used in association are extremely important to clarify possible false results, essential for reducing the risk of post-transfusion contamination.

Author Biography

Alexandre Ehrhardt, ULBRA - Universidade Luterana do Brasil - campus Carazinho. Br 285, Km 335. Carazinho , RS 99500-000 FASURGS - Faculdade Especializada na Área da Saúde do Rio Grande do Sul Rua Angélica Oto, 160. Boqueirão. Passo Fundo, RS 99025-270

Docente dos curso de Graduação - Biomedicina e Enfermagem - ULBRA

Perito Cadastrado no Tribunal de Justiça do Rio Grande do Sul nas áreas de Análises Clínicas e Toxicológicas

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Published

2020-12-08

How to Cite

Carboni, G., Boehm, R. E., Doro, C. M., Tiecher, C., & Ehrhardt, A. (2020). COMPARATIVE ANALYSIS OF METHODS FOR HCV SCREENING IN HEMOTHERAPY SERVICES. Infarma - Pharmaceutical Sciences, 32(4), 336–343. https://doi.org/10.14450/2318-9312.v32.e4.a2020.pp336-343

Issue

Vol. 32 No. 4 (2020)

Section

Original article

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