Cordia verbenacea DC and Mikania sp.: INTERFERENCE IN THE MICROBIOLOGICAL QUALITY CONTROL ASSAY

Authors

  • Catarina Bernardes Pereira Laboratório de Controle da Qualidade, Faculdade de Ciências da Saúde, Universidade de Brasília, Campus Darcy Ribeiro, Asa Norte, Brasília-DF, Brazil. CEP 70910-900.
  • Suelen de Castro Fonseca Laboratório de Controle da Qualidade, Faculdade de Ciências da Saúde, Universidade de Brasília, Campus Darcy Ribeiro, Asa Norte, Brasília-DF, Brazil. CEP 70910-900.
  • Lorena Ferreira Gomes Laboratório de Controle da Qualidade, Faculdade de Ciências da Saúde, Universidade de Brasília, Campus Darcy Ribeiro, Asa Norte, Brasília-DF, Brazil. CEP 70910-900.
  • Felipe Lipparelli Tironi Laboratório de Controle da Qualidade, Faculdade de Ciências da Saúde, Universidade de Brasília, Campus Darcy Ribeiro, Asa Norte, Brasília-DF, Brazil. CEP 70910-900. Núcleo de Farmácia Viva SES-DF, Avenida Sucupira S/Nº, Riacho Fundo- I, Brasília-DF, Brazil. CEP: 71825-300
  • Nilton L. Neto Júnior Núcleo de Farmácia Viva SES-DF, Avenida Sucupira S/Nº, Riacho Fundo- I, Brasília-DF, Brazil. CEP: 71825-300
  • Christopher William Fagg Faculdade de Ceilândia, Universidade de Brasília, Centro Metropolitano, conjunto A, lote 01, Brasília – DF, Brazil. CEP: 72220-900
  • Luiz Alberto Simeoni Laboratório de Farmacologia Molecular, Faculdade de Ciências da Saúde, Universidade de Brasília, Campus Darcy Ribeiro, Asa Norte, Brasília-DF, Brazil. CEP 70910-900.
  • Pérola Oliveira Magalhães Laboratório de Controle da Qualidade, Faculdade de Ciências da Saúde, Universidade de Brasília, Campus Darcy Ribeiro, Asa Norte, Brasília-DF, Brazil. CEP 70910-900.
  • Dâmaris Silveira Laboratório de Controle da Qualidade, Faculdade de Ciências da Saúde, Universidade de Brasília, Campus Darcy Ribeiro, Asa Norte, Brasília-DF, Brazil. CEP 70910-900.
  • Yris Maria Fonseca-Bazzo Faculdade de Ciências da Saúde - Universidade de Brasília

DOI:

https://doi.org/10.14450/2318-9312.v26.e2.a2014.pp111-118

Keywords:

guaco, erva baleeira, Escherichia coli, Candida albicans, microbiological techniques

Abstract

This study aims to evaluate the microbial contamination of Mikania sp. (plant drug, tincture and syrup) and Cordia verbenacea (plant drug, tincture and ointment). Furthermore, it had been investigated the possible interference caused by secondary metabolites from these species on proposed evaluation. The microbial contamination was determined using the pour plate method. The evaluation of the interference in the process of the microbiological quality was performed by determining the recovery of viable microorganisms using Escherichia coli (ATCC 25922) and Candida albicans (ATCC 90028). In the evaluation the microbiological quality, dried leaves from C. verbenacea and Mikania sp. showed 4.5 x 104 CFU/g and 2.08 x 104 CFU/g of bacteria, respectively. Regarding count of C. verbenacea tincture, was found 3.3 x 103 CFU/mL for yeasts and molds and 2.2 x 103 CFU/ mL for bacteria. For the ointment containing C. verbenacea tincture was found 1.1 x 103 CFU/g of bacteria, while fungi were not detected. Regarding microbial contamination of the syrup was not observed growth of bacteria, molds and yeasts in any of  repetitions. Also, derivatives from both species were able to inhibit the growth of strains of E. coli and C. albicans. Thus, the presence of secondary metabolites with antimicrobial activity on C. verbenacea and Mikania sp. seems to interfere in the evaluation of the microbiological quality of products derived from plant drug.

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Published

2014-06-27

How to Cite

Pereira, C. B., Fonseca, S. de C., Gomes, L. F., Tironi, F. L., Neto Júnior, N. L., Fagg, C. W., … Fonseca-Bazzo, Y. M. (2014). Cordia verbenacea DC and Mikania sp.: INTERFERENCE IN THE MICROBIOLOGICAL QUALITY CONTROL ASSAY. Infarma - Pharmaceutical Sciences, 26(2), 111–118. https://doi.org/10.14450/2318-9312.v26.e2.a2014.pp111-118

Issue

Vol. 26 No. 2 (2014)

Section

Original article

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